The Application of DEAE-cellulose Column Chromatography to the

This should not be done for a column packed with DEAE Sephadex A-25, QAE Buffers for anion exchange chromatography pH interval Substance Conc

At pH , albumin, with a pI of , is negatively charged; immunoglobulin with a pI ∼8 is positively charged If the mixture is applied to a DEAE column, the

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deae ceramic hyperd Anion-exchange chromatography is a universal purification step, which takes advantage of the highly acidic pI of brachyurins Acid